模型信息
中文名称:HIV-1 ADA腹腔途径感染CD34-NSG小鼠模型
英文名称:HIV-1 ADA intraperitoneally infected CD34-NSG mouse model
类型:艾滋病毒动物模型
分级:NA
用途:用于艾滋病相关研究
研制单位:内布拉斯加大学医学中心
保存单位:内布拉斯加大学医学中心
研究背景
来源文献:Human Interleukin-34 facilitates microglia-like cell differentiation and persistent HIV-1 infection in humanized mice
PMID:30832693
DOI:10.1186/s13024-019-0311-y
制备方法
Mice with established human hemato-lymphoid reconstitution (∼6–8 months of age) and positive for IL-34 expression were intraperitoneally infected (n =12) with the 104 tissue culture infectious dose50 (TCID50) of macrophage-tropic HIV-1ADA strain.NOG (formally, NOD.Cg-Prkdcscidil2gtmlSug/Jic) and NOD/ShiJcl (NOD) were used. NOG mice were maintained in the CIEA under specific-pathogen-free conditions. NOD mice were purchased from CIEA (Tokyo,Japan). To generate the hIL-34 expressing transgenic NOG mouse (NOG-hIL-34), a linearized DNA vector (pCMV6-XL4) containing human IL-34 cDNA (Origene Technologies, Inc., Rockville, MD, USA) under the control of a CMV promoter, was microinjected into fertilized eggs obtained by mating NOG and NOD mice.
评价验证
诊断:病毒在血液中复制。
生物安全性
所有感染动物必须在ABSL-3室中进行。
讨论与结论
1.该模型制备比较成熟,国内外都有此模型,构建和评价指标一致,所以该模型应用广泛,具有统一的操作规程。
2. 该模型制备的关键:1)小鼠必须是重度免疫缺陷的,而且饲养环境必须洁净;2)HIV操作需要生物安全。
3.此模型具有完善的人的免疫系统,HIV感染后,具有艾滋病病人相似的临床症状,所以具有很高的比较医学应用价值。
了解中析
实验室仪器
合作客户
